Polymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum

Recombinant DNA probes and polymerase chain reaction for detection of Mycoplasma gallisepticum strains.

Two recombinant DNA clones, pMG286.2 and pMG301.1, were isolated from the partial genomic library of Mycoplasma gallisepticum strain S6. Recombinant M. gallisepticum specific fragments were used as probes in Southern hybridisation with 10 M. gallisepticum strains whose DNA was digested by EcoRI, HindIII, BglII, RsaI and BamHI. The 1.5 kb fragment pMG301.1 did not show polymorphism in hybridisat...

Detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction

Five common tetracycline resistance genes tet(A), tet(B), tet(M), tet(O) and tet(S) were studied by polymerase chain reaction in 100 bacteria isolated from Iranian fish farms. In the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. Nine isolates out of 46 Aeromonas spp. contained eithe...

Detection of Babesia ovis Using Polymerase Chain Reaction.

Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

Detection of Mycoplasma pneumoniae by using the polymerase chain reaction.

The polymerase chain reaction (PCR) technique was used to detect Mycoplasma pneumoniae. A specific DNA sequence for M. pneumoniae was selected from a genomic library, and two oligonucleotides were chosen in this sequence to give an amplified fragment of 144 base pairs. We show that DNA from different M. pneumoniae strains can be detected by PCR, with DNA from other Mycoplasma species giving neg...

Polymerase chain reaction-based discrimination of viable from non-viable Mycoplasma gallisepticum.

The present study was based on the reverse transcription polymerase chain reaction (RT-PCR) of the 16S ribosomal nucleic acid (rRNA) of Mycoplasma for detection of viable Mycoplasma gallisepticum. To determine the stability of M. gallisepticum 16S rRNA in vitro, three inactivation methods were used and the suspensions were stored at different temperatures. The 16S rRNA of M. gallisepticum was d...